C4BPB is a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C.1) Anderson, N.L., et al., (2004) Mol. Cell Proteomics 3 (4), 311-326.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human C4BPB.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
C4BPB antibody can be used for detection of C4BPB by ELISA at 1:12500. C4BPB antibody can be used for detection of C4BPB by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Application
Antibody used in WB on Human 721_B at 1 ug/ml.
Application Notes
C4BPB antibody can be used for detection of C4BPB by ELISA at 1:12500. C4BPB antibody can be used for detection of C4BPB by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store C4BPB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.