Application
Immunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.Application
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 2 μg/mL.Application
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.Application
Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 μg/mL.Application
Western blot analysis of CAD in mouse kidney tissue lysate with CAD antibody at 2 μg/mL.| Product Name | CAD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.1) Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 |
| Immunogen | CAD antibody was raised against a peptide corresponding to 17 amino acids near the center of murine CAD. The immunogen is located within amino acids 190 - 240 of CAD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CAD antibody can be used for detection of CAD by Western blot at 2 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Dffb |
|---|---|
| Alternative Names | CAD Antibody: CAD CPAN 40kDa DFF40 Didff 5730477D02Rik Cad DNA fragmentation factor subunit beta Caspase-activated deoxyribonuclease CAD |
| Molecular Weight(MW) | 40 kDa |
Application
Immunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.
Application
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 2 μg/mL.
Application
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.
Application
Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 μg/mL.
Application
Western blot analysis of CAD in mouse kidney tissue lysate with CAD antibody at 2 μg/mL.| Application Notes | CAD antibody can be used for detection of CAD by Western blot at 2 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | CAD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CAD Antibody is supplied in PBS containing 0.02% sodium azide. |
