Application
Confocal immunofluorescent analysis of CALCR Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of lysate from MCF-7, A431 cell line, using CALCR Antibody with(+) or without(-) peptides at 1:1000 at each lane. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.| Product Name | CALCR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CALCR is a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. The encoded protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis.1) Nussenzveig, D.R., et al. Endocrinology 136(5):2047-2051(1995) Nakamura, M., et al. Biochem. Biophys. Res. Commun. 209(2):744-751(1995) Egerton, M., et al. J. Mol. Endocrinol. 14(2):179-189(1995) Gorn, A.H., et al. J. Clin. Invest. 90(5):1726-1735(1992) |
| Immunogen | This CALCR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human CALCR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CALCR |
|---|---|
| Alternative Names | Calcitonin receptor CT-R CALCR |
| Molecular Weight(MW) | 59 kDa |
Application
Confocal immunofluorescent analysis of CALCR Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of lysate from MCF-7, A431 cell line, using CALCR Antibody with(+) or without(-) peptides at 1:1000 at each lane. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
