Application
Antibody used in WB on Human Daudi cells at 1.0-2.0 ug/ml.| Product Name | CALR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Calreticulin is a multifunctional protein that acts as a major Ca (2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.1) Urade, R., et al., (2004) Biochemistry 43 (27), 8858-8868. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CALR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CALR antibody can be used for detection of CALR by ELISA at 1:12500. CALR antibody can be used for detection of CALR by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CALR |
|---|---|
| Alternative Names | CALR RO CRT SSA cC1qR HEL-S-99n |
| Molecular Weight(MW) | 48 kDa |
Application
Antibody used in WB on Human Daudi cells at 1.0-2.0 ug/ml.| Application Notes | CALR antibody can be used for detection of CALR by ELISA at 1:12500. CALR antibody can be used for detection of CALR by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Form | Liquid |
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| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CALR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
