Application
Western blot analysis of extracts from K562 cells treated with H2O2 using Phospho-CaMK4 (Thr196/200) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | CaMK4 (phospho Thr196/200) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2).1) Oury F, et al. (2010)Genes Dev 24, 2330-42. Dias WB, Cheung WD, Wang Z, Hart GW (2009) J Biol Chem 284, 21327-37. Chow FA, Anderson KA, Noeldner PK, Means AR (2005)J Biol Chem 280, 20530-8. |
| Immunogen | CaMK4 (phospho Thr196/200) antibody was raised against a peptide sequence around phosphorylation site of threonine 200 (M-K-T (p)-V-C) derived from Human CaMK4 or threonine 196 (M-K-T (p)-V-C) derived from Mouse CaMK4. |
| Modification | p-Thr196/200 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAMK4 |
|---|---|
| Alternative Names | CAM kinase-GR CAMK4 CaMK IV Calspermin KCC4 |
| Molecular Weight(MW) | 60 kDa |
| Entrez Gene | 814 |
|---|---|
| Protein Accession | NP_001735.1 |
Application
Western blot analysis of extracts from K562 cells treated with H2O2 using Phospho-CaMK4 (Thr196/200) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
