Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMKK2 antibody (Cat.#AP711b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of CAMKK2 using rabbit polyclonal CAMKK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMKK2 gene (Lane 2).| Product Name | CAMKK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CAMKK2 belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. CAMKK2 appears to be involved in hippocampal activation of CREB1.1) Hsu, L.S., et al., J. Biol. Chem. 276(33):31113-31123 (2001). |
| Immunogen | This CAMKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483-512 amino acids from the C-terminal region of human CAMKK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CAMKK2 |
|---|---|
| Alternative Names | Calcium/calmodulin-dependent protein kinase kinase 2 CaM-KK 2 CaM-kinase kinase 2 CaMKK 2 Calcium/calmodulin-dependent protein kinase kinase beta CaM-KK beta CaM-kinase kinase beta CaMKK beta CAMKK2 CAMKKB KIAA0787 |
| Molecular Weight(MW) | 65 kDa |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMKK2 antibody (Cat.#AP711b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of CAMKK2 using rabbit polyclonal CAMKK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMKK2 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
