Application
WB analysis of extracts from untreated and Calyculin A-treated HeLa cells using PTEN (phospho Ser370) antibody.Application
IHC-P analysis of human breast carcinoma using PTEN (phospho Ser370) antibody.Application
ICC/IF analysis of methanol-fixed MCF7 cells using PTEN (phospho Ser370) antibody.| Product Name | PTEN (phospho Ser370) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTEN (phospho Ser370)This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S370 of human PTEN |
| Modification | p-Ser370 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:100 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PTEN1 MHAM TEP1 CWS1 10q23del MMAC1 BZS DEC phosphatase and tensin homolog PTEN GLM2 |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) |
Application
WB analysis of extracts from untreated and Calyculin A-treated HeLa cells using PTEN (phospho Ser370) antibody.
Application
IHC-P analysis of human breast carcinoma using PTEN (phospho Ser370) antibody.
Application
ICC/IF analysis of methanol-fixed MCF7 cells using PTEN (phospho Ser370) antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
