Application
Western blot analysis of Carabin in human spleen tissue lysate with Carabin antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 μg/mL.| Product Name | Carabin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Carabin Antibody: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.1) Weil R and Israel A. Deciphering the pathway from the TCR to NF-kappaB. Cell Death Differ.2006; 13:826-33. |
| Immunogen | Carabin antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Carabin. The immunogen is located within amino acids 100 - 150 of Carabin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBC1D10C |
|---|---|
| Alternative Names | Carabin Antibody: EPI64C CARABIN Carabin TBC1 domain family member 10C |
Application
Western blot analysis of Carabin in human spleen tissue lysate with Carabin antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 μg/mL.| Application Notes | Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Carabin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Carabin Antibody is supplied in PBS containing 0.02% sodium azide. |
