Application
Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 μg /ml.Application
Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 μg/mL.| Product Name | CARD9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CARD9 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-κB. CARD9 is an upstream activator of BCL-10 and NF-κB signaling.1) Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL, Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefano PS, Alnemri ES. CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-κ B. J Biol Chem. 2000;275(52):41082-6. (RD0202) |
| Immunogen | CARD9 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CARD9. The immunogen is located within the last 50 amino acids of CARD9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| CARD9 antibody can be used for detection of CARD9 by Western blot at 2.5 μg/mL. A band at approximately 59 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CARD9 |
|---|---|
| Alternative Names | CARD9 Antibody: CANDF2 hCARD9 Caspase recruitment domain-containing protein 9 |
| Molecular Weight(MW) | 59 kDa |
Application
Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 μg /ml.
Application
Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 μg/mL.| Application Notes | CARD9 antibody can be used for detection of CARD9 by Western blot at 2.5 μg/mL. A band at approximately 59 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CARD9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CARD9 Antibody is supplied in PBS containing 0.02% sodium azide. |
