Application
Immunofluorescence of CARM1 in Jurkat cells with CARM1 antibody at 5 μg/mL.Application
Western blot analysis of CARM1 in Jurkat cell lysate with CARM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | CARM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CARM1 Antibody: Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation. CARM1 functions as a transcriptional co-activator for various nuclear receptors and NF-κB. It has also been shown to methylate histone H3 arginines, inhibiting the binding of corepressors and protecting chromatin from deacetylation, thereby facilitating transcription.1) Frankel A, Yadav N, Lee J, et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J. Biol. Chem. 2002; 277:3537-43. |
| Immunogen | Rabbit polyclonal CARM1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CARM1. The immunogen is located within amino acids 470 - 520 of CARM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CARM1 antibody can be used for detection of CARM1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CARM1 |
|---|---|
| Alternative Names | CARM1 Antibody: PRMT4 PRMT4 Histone-arginine methyltransferase CARM1 Coactivator-associated arginine methyltransferase 1 |
| Molecular Weight(MW) | Predicted: 67 kDa |
Application
Immunofluorescence of CARM1 in Jurkat cells with CARM1 antibody at 5 μg/mL.
Application
Western blot analysis of CARM1 in Jurkat cell lysate with CARM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Application Notes | CARM1 antibody can be used for detection of CARM1 by Western blot at 1 - 2 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CARM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CARM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
