Application
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/pS370) antibody diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)Application
PTEN (phospho Thr366/Ser370) antibody detects PTEN (phospho Thr366/Ser370) protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTEN (phospho Thr366/Ser370) protein stained by PTEN (phospho Thr366/Ser370) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | PTEN (phospho Thr366/Ser370) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTEN (phospho Thr366/pS370) (phosphatase and tensin homolog)This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PTEN (phospho Thr366/Ser370). The exact sequence is proprietary. |
| Modification | p-Thr366/Ser370 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Rhesus Monkey, BovineMouse (100%), Rat (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PTEN BZS phosphatase and tensin homolog PTEN1 CWS1 10q23del GLM2 TEP1 MMAC1 MHAM DEC |
|---|---|
| Target | PTEN (phospho Thr366/Ser370) |
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/pS370) antibody diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)
Application
PTEN (phospho Thr366/Ser370) antibody detects PTEN (phospho Thr366/Ser370) protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTEN (phospho Thr366/Ser370) protein stained by PTEN (phospho Thr366/Ser370) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A431 , A431 (CIP) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
