Application
Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 μg/mL.Application
Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | CASK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASK Antibody: CMG, FGS4, LIN2, TNRC8, CAGH39, CAMGUK, MICPCH, MRXSNA, Peripheral plasma membrane protein CASK, Protein lin-2 homolog, hCASK |
| Product description | CASK Antibody: CASK (Calcium/calmodulin-dependent serine protein kinase), a conserved multi-domain scaffolding protein, belongs to a MAGUK (membrane-associated guanylate kinase homologs) subfamily and is involved in cell junction organization, tumor suppression and signaling. It is characterized by a novel domain structure that consists of a calcium/calmodulin-dependent protein kinase domain followed by PDZ, SH3 and guanylate kinase-like (GUK) domains. CASK is ubiquitously expressed and significantly greater in brain where it is thought to be involved in signaling at neuronal synapses. CASK interacts with CASKINs and defects in CASK are the cause of mental retardation X-linked CASK-related.1) Dimitratos SD, Woods DF, and Bryant PJ. Camguk, Lin-2, and CASK: novel membrane associated guanylate kinase homologs that also contain CaM kinase domains. Mech. Dev. 1997; 63:127-30. |
| Immunogen | CASK antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CASK. The immunogen is located within amino acids 760 - 810 of CASK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CASK antibody can be used for detection of CASK by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASK |
|---|---|
| Alternative Names | CASK Antibody: CMG FGS4 LIN2 TNRC8 CAGH39 CAMGUK MICPCH MRXSNA Peripheral plasma membrane protein CASK Protein lin-2 homolog hCASK |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least six alternatively spliced transcript variants have been observed. |
Application
Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 μg/mL.
Application
Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | CASK antibody can be used for detection of CASK by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CASK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CASK Antibody is supplied in PBS containing 0.02% sodium azide. |
