Application
Immunohistochemistry of CASR in human kidney tissue with CASR antibody at 5 μg/mL.Application
Immunofluorescence of CASR in human kidney tissue with CASR antibody at 20 μg/mL.Application
Western blot analysis of CASR in EL4 cell lysate with CASR antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | CASR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASR Antibody: CAR, FHH, FIH, HHC, EIG8, HHC1, NSHPT, PCAR1, GPRC2A, HYPOC1, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, CaSR |
| Product description | The calcium-sensing receptor protein (CASR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (1). It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis (1). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (1,2). Recent evidence suggests that activated CASR contributes to the cytokine secretion through the partial MAPK and NF-kappaB pathways in T cells (3).1) Pollak MR, Brown EM, Chou YH, et al. Mutations in the human Ca(2+)-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. Cell 1993; 75:1297-303. |
| Immunogen | CASR antibody was raised against a 15 amino acid peptide near the amino terminus of human CASR. The immunogen is located within the first 50 amino acids of CASR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CASR antibody can be used for detection of CASR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASR |
|---|---|
| Alternative Names | CASR Antibody: CAR FHH FIH HHC EIG8 HHC1 NSHPT PCAR1 GPRC2A HYPOC1 Extracellular calcium-sensing receptor Parathyroid cell calcium-sensing receptor 1 CaSR |
| Molecular Weight(MW) | Predicted: 101, 120 kDa Observed: 115, 125 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | CASR antibody is human, mouse and rat reactive. At least two isoforms of CASR are known to exist. |
| Entrez Gene | 846 |
|---|---|
| Protein Accession | NP_001171536 |
Application
Immunohistochemistry of CASR in human kidney tissue with CASR antibody at 5 μg/mL.
Application
Immunofluorescence of CASR in human kidney tissue with CASR antibody at 20 μg/mL.
Application
Western blot analysis of CASR in EL4 cell lysate with CASR antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | CASR antibody can be used for detection of CASR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CASR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CASR Antibody is supplied in PBS containing 0.02% sodium azide. |
