Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CAT Antibody in mouse kidney tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CAT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CAT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Catalase, CAT |
| Product description | CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. It promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.1) Oh,J.H., et.al., Mamm. Genome 16 (12), 942-954 (2005) |
| Immunogen | This CAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the Central region of human CAT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAT |
|---|---|
| Alternative Names | Catalase CAT |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CAT Antibody in mouse kidney tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CAT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
