Application
Flow cytometric analysis of HepG2 cells using CATB Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CATB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of CATB Antibody in Y79 cell line lysates (35ug/lane)| Product Name | CATB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cathepsin B, APP secretase, APPS, Cathepsin B1, Cathepsin B light chain, Cathepsin B heavy chain, CTSB, CPSB |
| Product description | The protein CATB is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors.1) Zhang,H., J. Immunol. 182 (11), 6993-7000 (2009) |
| Immunogen | This CATB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the Central region of human CATB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTSB |
|---|---|
| Alternative Names | Cathepsin B APP secretase APPS Cathepsin B1 Cathepsin B light chain Cathepsin B heavy chain CTSB CPSB |
| Molecular Weight(MW) | 38 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of HepG2 cells using CATB Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CATB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of CATB Antibody in Y79 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
