Application
Western blot analysis of lysed extracts from HT29 and HUVEC cells using Caveolin-1 (Ab-14).| Product Name | Caveolin 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CGL3, PPH3, BSCL3, VIP21, MSTP085, CAV, CAV1 |
| Product description | The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.1) Zhang Y, et al. (2005) Mol Cell Proteomics. 4 (9): 1240-1250. Labrecque L, et al. (2004) J Biol Chem. 279 (50): 52132-52140. Fielding PE, et al. (2004) Biochemistry. 43 (9): 2578-2586. Labrecque L, et al. (2003) Mol Biol Cell. 14 (1): 334-347. Maggi D, et al. (2002) Biochem Biophys Res Commun. 295 (5): 1085-1089. |
| Immunogen | Caveolin-1 (Ab-14) antibody was raised against a peptide sequence around aa.12~16 (H-L-Y-T-V) derived from Human CAVEOLIN-1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAV1 |
|---|---|
| Alternative Names | CGL3 PPH3 BSCL3 VIP21 MSTP085 CAV CAV1 |
| Molecular Weight(MW) | 24 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Caveolin-1 protein. |
| Entrez Gene | 857 |
|---|---|
| Protein Accession | NP_001166366.1 |
Application
Western blot analysis of lysed extracts from HT29 and HUVEC cells using Caveolin-1 (Ab-14).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
