Application
Immunofluorescence of cbl in human lymph node tissue with cbl antibody at 20 μg/mL.Application
Western blot analysis of cbl in Daudi cell lysate with cbl antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Cbl Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cbl Antibody: CBL2, NSLL, C-CBL, RNF55, FRA11B, CBL2, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene |
| Product description | Cbl Antibody: The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.1) Thien CBF and Langdon WY. C-Cbl and Cbl-b ubiquitin ligases: substrate diversity and the negative regulation of signaling responses. Biochem. J. 2005; 391:153-66 |
| Immunogen | Cbl antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human cbl. The immunogen is located within amino acids 810 - 860 of Cbl. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Cbl antibody can be used for detection of cbl by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBL |
|---|---|
| Alternative Names | Cbl Antibody: CBL2 NSLL C-CBL RNF55 FRA11B CBL2 E3 ubiquitin-protein ligase CBL Casitas B-lineage lymphoma proto-oncogene |
| Sequence Similarities | 3) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Application
Immunofluorescence of cbl in human lymph node tissue with cbl antibody at 20 μg/mL.
Application
Western blot analysis of cbl in Daudi cell lysate with cbl antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | Cbl antibody can be used for detection of cbl by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Cbl antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Cbl Antibody is supplied in PBS containing 0.02% sodium azide. |
