Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Antibody is used in Western blot to detect CBX8 in mouse kidney tissue lysate.| Product Name | CBX8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Chromobox protein homolog 8, Polycomb 3 homolog, Pc3, hPc3, Rectachrome 1, CBX8, PC3, RC1 |
| Product description | Chromobox homolog 8 (CBX8 or Pc3) is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators and thus are essential for proper development. Changes in expression of PcG proteins have been associated with cancer. CBX8 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility. Structurally, CBX4 contains 1 chromo domain, which is 40 to 50 amino acids long.1) Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). |
| Immunogen | This CBX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-337 amino acids from the C-terminal region of human CBX8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBX8 |
|---|---|
| Alternative Names | Chromobox protein homolog 8 Polycomb 3 homolog Pc3 hPc3 Rectachrome 1 CBX8 PC3 RC1 |
| Molecular Weight(MW) | 43 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Antibody is used in Western blot to detect CBX8 in mouse kidney tissue lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
