Application
Immunofluorescence of CCL4 in rat brain tissue with CCL4 antibody at 20 μg/ml.Application
Immunohistochemistry of CCL4 in rat brain tissue with CCL4 antibody at 2.5 μg/ml.Application
Western blot analysis of CCL4 in rat brain tissue lysate with CCL4 antibody at 1 μg/mL.| Product Name | CCL4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCL4 Antibody: ACT2, G-26, HC21, LAG1, LAG-1, MIP1B, SCYA2, SCYA4, MIP1B1, AT744.1, MIP-1-beta, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein |
| Product description | CCL4 Antibody: CCL4, also known as macrophage inflammatory protein 1-beta (MIP1B), belongs to the intercrine beta (chemokine CC) family. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ; with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells.1) Baixeras E, Roman-Roman S, Jitsukawa S, et al. Cloning and expression of a lymphocyte activation gene (LAG-1). Mol. Immunol. 1990; 27:1091-102. |
| Immunogen | Rabbit polyclonal CCL4 antibody was raised against a 15 amino acid peptide near the center of human CCL4. The immunogen is located within the first 50 amino acids of CCL4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CCL4 antibody can be used for detection of CCL4 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL4 |
|---|---|
| Alternative Names | CCL4 Antibody: ACT2 G-26 HC21 LAG1 LAG-1 MIP1B SCYA2 SCYA4 MIP1B1 AT744.1 MIP-1-beta C-C motif chemokine 4 G-26 T-lymphocyte-secreted protein |
| Molecular Weight(MW) | Predicted: 10 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (80%) |
Application
Immunofluorescence of CCL4 in rat brain tissue with CCL4 antibody at 20 μg/ml.
Application
Immunohistochemistry of CCL4 in rat brain tissue with CCL4 antibody at 2.5 μg/ml.
Application
Western blot analysis of CCL4 in rat brain tissue lysate with CCL4 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | CCL4 antibody can be used for detection of CCL4 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCL4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CCL4 Antibody is supplied in PBS containing 0.02% sodium azide. |
