Recently, it has been shown that genetic polymorphisms can result in diminished expression of CCL5, which results in increased susceptibility to and progression of infectious diseases. CCL5, together with Th cytokine mRNA expression, is temporally up-regulated during pneumococcal carriage. CCL5 is an essential factor for the induction and maintenance of protective pneumococcal immunity1) Palaniappan, R., et al., (2006) J. Immunol. 176 (4), 2346-2356.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCL5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CCL5 antibody can be used for detection of CCL5 by ELISA at 1:62500. CCL5 antibody can be used for detection of CCL5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
CCL5 antibody can be used for detection of CCL5 by ELISA at 1:62500. CCL5 antibody can be used for detection of CCL5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CCL5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt