CCL7 encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.1) Jang, M.K., et al., (2004) Biochem. Biophys. Res. Commun. 323 (3), 898-905.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCL7.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
CCL7 antibody can be used for detection of CCL7 by ELISA at 1:62500. CCL7 antibody can be used for detection of CCL7 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
1) Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate
Application Notes
CCL7 antibody can be used for detection of CCL7 by ELISA at 1:62500. CCL7 antibody can be used for detection of CCL7 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CCL7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.