Application
PTK9 antibody detects PTK9 protein at cytoplasm on SW480 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SW480 xenograft. PTK9 antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTK9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Product Name | PTK9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTK9 (twinfilin, actin-binding protein, homolog 1 (Drosophila))This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PTK9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, BovineRat (94%), Chicken (90%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | A6 antibody MGC23788 antibody MGC41876 antibody PTK9 antibody TWF1 antibody A6 protein tyrosine kinase antibody PTK9 protein tyrosine kinase 9 antibody twinfilin-1 antibody protein A6 antibody protein tyrosine kinase 9 antibody "twinfilin actin- |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) |
| Cellular Localization | Cytoplasm , cytoskeleton (By similarity) |
Application
PTK9 antibody detects PTK9 protein at cytoplasm on SW480 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SW480 xenograft. PTK9 antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTK9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Positive Control | 293T , A431 , H1299 , HeLaS3 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
