Application
Antibody used in WB on Human HEK 293T at 1:1000 dilution.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on Rat Hippocampal Neurons at 1:200.Application
Antibody used in WB on Human HEK293T at: 1:1000 (Lane 1: Negative control (anti-FLAG antibody), Lane 2: Immunoprecipitation with CCT4 (C-term), Lane 3: 10 ug lysate).Application
Antibody used in WB on Human 293T.| Product Name | CCT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCT4, SRB, Cctd, CCT-DELTA |
| Product description | CCT4 is a subunit of a cytosolic hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55.1) Kimura, K., et al., (2006) Genome Res. 16 (1), 55-65. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCT4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CCT4 antibody can be used for detection of CCT4 by ELISA at 1:312500. CCT4 antibody can be used for detection of CCT4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CCT4 |
|---|---|
| Alternative Names | CCT4 SRB Cctd CCT-DELTA |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human HEK 293T at 1:1000 dilution.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Rat Hippocampal Neurons at 1:200.
Application
Antibody used in WB on Human HEK293T at: 1:1000 (Lane 1: Negative control (anti-FLAG antibody), Lane 2: Immunoprecipitation with CCT4 (C-term), Lane 3: 10 ug lysate).
Application
Antibody used in WB on Human 293T.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | CCT4 antibody can be used for detection of CCT4 by ELISA at 1:312500. CCT4 antibody can be used for detection of CCT4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CCT4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
