Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: A673 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit| Product Name | CCT8L2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Putative T-complex protein 1 subunit theta-like 2, CCT8L2, CESK1 |
| Product description | Possible molecular chaperone; assists the folding of proteins upon ATP hydrolysis.1) Riazi M.A.,et al.Genomics 62:90-94(1999). |
| Immunogen | This CCT8L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-124 amino acids from human CCT8L2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCT8L2 |
|---|---|
| Alternative Names | Putative T-complex protein 1 subunit theta-like 2 CCT8L2 CESK1 |
| Molecular Weight(MW) | 59 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: A673 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
