Application
Flow cytometric analysis of NCI-H292 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of in mouse heart tissue lysates (35ug/lane)Application
immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.| Product Name | CD105 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endoglin, CD105, ENG, END |
| Product description | CD105 is a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in its gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.1) Chen,Y., Ann. Neurol. 66 (1), 19-27 (2009) |
| Immunogen | This CD105 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-409 amino acids from the Central region of human CD105. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | ENG |
|---|---|
| Alternative Names | Endoglin CD105 ENG END |
| Molecular Weight(MW) | 71 kDa |
Application
Flow cytometric analysis of NCI-H292 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of in mouse heart tissue lysates (35ug/lane)
Application
immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
