Application
Western blot analysis of PTP1B (pS50) expression in COLO205 (A); human muscle (B); mouse heart (C) whole cell lysates.Application
Immunohistochemical analysis of PTP1B (pS50) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | PTP1B (phospho Ser50) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTP1B (phospho Ser50)The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PTP1B (pS50). The exact sequence is proprietary. |
| Modification | p-Ser50 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PTPN1 PTP1B protein tyrosine phosphatase non-receptor type 1 |
|---|---|
| Target | PTP1B |
| Tissue Specificity | Recognizes endogenous levels of PTP1B (pS50) protein. |
Application
Western blot analysis of PTP1B (pS50) expression in COLO205 (A); human muscle (B); mouse heart (C) whole cell lysates.
Application
Immunohistochemical analysis of PTP1B (pS50) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
