Application
Sample(30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:500Application
PTP1B antibody [N1N3] detects PTP1B protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. PTP1B antibody [N1N3] dilution: 1:500.| Product Name | PTP1B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PTP1B (protein tyrosine phosphatase, non-receptor type 1)The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PTP1B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (94%), Rat (95%), Zebrafish (86%), Xenopus laevis (87%), Pig (99%), Chicken (96%), Rhesus Monkey (100%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PTP1B antibody PTPN1 antibody tyrosine-protein phosphatase non-receptor type 1 antibody protein-tyrosine phosphatase 1B antibody protein tyrosine phosphatase 1B antibody "protein tyrosine phosphatase placental antibody" "protein tyrosine phosphatase |
|---|---|
| Target | PTP1B |
| Molecular Weight(MW) | 50 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Sample(30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:500
Application
PTP1B antibody [N1N3] detects PTP1B protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. PTP1B antibody [N1N3] dilution: 1:500.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
