Application
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using PTPN12 antibodyApplication
Immunofluorescence analysis of methanol-fixed HeLa, using PTPN12 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using PTPN12 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PTPN12 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:5000| Product Name | PTPN12 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTPN12 (protein tyrosine phosphatase, non-receptor type 12)The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PTPN12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, BovineMouse (99%), Xenopus laevis (81%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PTP-PEST antibody PTPG1 antibody PTPN12 antibody tyrosine-protein phosphatase non-receptor type 12 antibody protein-tyrosine phosphatase G1 antibody "protein tyrosine phosphatase non-receptor type 12 antibody" |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using PTPN12 antibody
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PTPN12 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using PTPN12 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PTPN12 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:5000| Positive Control | A549 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
