Application
Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of CD166 antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | CD166 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD |
| Product description | CD166 is cell adhesion molecule that binds to CD6. The protein is involved in neurite extension by neurons via heterophilic and homophilic interactions. It may play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.1) Kahlert,C., Br. J. Cancer 101 (3), 457-464 (2009) |
| Immunogen | This CD166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human CD166. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ALCAM |
|---|---|
| Alternative Names | CD166 antigen Activated leukocyte cell adhesion molecule CD166 ALCAM MEMD |
| Molecular Weight(MW) | 65 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of CD166 antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
