Application
Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CD33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD33 Antibody: p67, SIGLEC3, SIGLEC-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3 |
| Product description | CD33 Antibody: CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid and lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 and SHP-2 and thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer's disease.1) Freeman SD, Kelm S, Barber EK, et al. Characterization of CD33 as a new member of the sialoadhesion family of cellular interaction molecules. Blood 1995; 85:2005-12. |
| Immunogen | CD33 antibody was raised against a 14 amino acid synthetic peptide near the center of human CD33. The immunogen is located within amino acids 180 - 230 of CD33. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD33 |
|---|---|
| Alternative Names | CD33 Antibody: p67 SIGLEC3 SIGLEC-3 Myeloid cell surface antigen CD33 Sialic acid-binding Ig-like lectin 3 Siglec-3 |
| Tissue Specificity | At least three isoforms of CD33 are known to exist; this antibody will detect all three isoforms. |
Application
Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CD33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CD33 Antibody is supplied in PBS containing 0.02% sodium azide. |
