Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of lysates from U266B1,HepG2 cell line,mouse brain tissue lysate(from left to right), using NT5E-M535 Antibody at 1:2000 at each lane. Lysates at 20ug per lane.Application
Western blot analysis of NT5E using rabbit polyclonal NT5E Antibody (M535) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.Application
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.Application
Western blot analysis of CD73 Pab in mouse brain tissue lysate| Product Name | CD73 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE |
| Product description | Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.1) Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003). Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002). Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990). Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988). Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002). |
| Immunogen | This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | NT5E |
|---|---|
| Alternative Names | 5'-nucleotidase 5'-NT Ecto-5'-nucleotidase CD73 NT5E NT5 NTE |
| Molecular Weight(MW) | 63 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of lysates from U266B1,HepG2 cell line,mouse brain tissue lysate(from left to right), using NT5E-M535 Antibody at 1:2000 at each lane. Lysates at 20ug per lane.
Application
Western blot analysis of NT5E using rabbit polyclonal NT5E Antibody (M535) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
Application
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Application
Western blot analysis of CD73 Pab in mouse brain tissue lysate| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
