Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 (Ab-15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using CDC2 (Ab-15).Application
Western blot analysis of lysed extracts from HeLa and MCF cells using CDC2 (Ab-15).| Product Name | CDC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC2, CDC28A, P34CDC2, MAPTL, MTBT1, TAU, CDC28, CDC2A, CDK1, Cyclin-dependent kinase 1 |
| Product description | Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.1) Y Gu, et al. (1992) EMBO J. 11 (11): 3995–4005. P Jin, et al. (1996) Cell Biology, Vol 134: 963-970, Tyagi, et al. (2005) Alpna1Carcinogenesis, Volume 26: 1978-1987 (10) Xiang S. et al. (1997) The EMBO Journal 16: 182–192 |
| Immunogen | CDC2 (Ab-15) antibody was raised against a peptide sequence around aa. 13~17 (G-T-Y-G-V) derived from Human CDC2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK1 |
|---|---|
| Alternative Names | CDC2 CDC28A P34CDC2 MAPTL MTBT1 TAU CDC28 CDC2A CDK1 Cyclin-dependent kinase 1 |
| Molecular Weight(MW) | 34 kDa |
| Tissue Specificity | This antibody detects endogenous level of total CDC2 protein. |
| Entrez Gene | 983 |
|---|---|
| Protein Accession | NP_001163877.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 (Ab-15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using CDC2 (Ab-15).
Application
Western blot analysis of lysed extracts from HeLa and MCF cells using CDC2 (Ab-15).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
