Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 (Phospho-Thr161).Application
Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161).| Product Name | CDC2 (phospho Thr161) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC2, CDC28A, P34CDC2, C1orf215, LAP18, OP18, CDC28, CDC2A, CDK1, Cyclin-dependent kinase 1 |
| Product description | Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.1) Ukomadu C, et al. (2003) J Biol Chem; 278 (7): 4840-6. Morris MC, et al. (2002) J Biol Chem; 277 (26): 23847-53. Brown NR, et al. (1999) J Biol Chem; 274 (13): 8746-56. Liu Y, et al. (2004) J Biol Chem; 279 (6): 4507-14. |
| Immunogen | CDC2 (Phospho-Thr161) antibody was raised against a peptide sequence around phosphorylation site of threonine161 (T-Y-T (p) -H-E) derived from Human CDC2. |
| Modification | p-Thr161 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK1 |
|---|---|
| Alternative Names | CDC2 CDC28A P34CDC2 C1orf215 LAP18 OP18 CDC28 CDC2A CDK1 Cyclin-dependent kinase 1 |
| Molecular Weight(MW) | 34 kDa |
| Tissue Specificity | This antibody detects endogenous level of CDC2 only when phosphorylated at threonine 161. |
| Entrez Gene | 983 |
|---|---|
| Protein Accession | NP_001163877.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 (Phospho-Thr161).
Application
Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
