Application
Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Tyr15).| Product Name | CDC2 (phospho Tyr15) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC2, CDC28A, P34CDC2, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, CDC28, CDC2A, CDK1, Cyclin-dependent kinase 1 |
| Product description | Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.1) Y Gu, et al. (1992) EMBO J. 11 (11): 3995–4005. P Jin, et al. (1996) Cell Biology, Vol 134: 963-970, Tyagi, et al. (2005) Alpna1Carcinogenesis, Volume 26: 1978-1987 (10) Xiang S. et al. (1997) The EMBO Journal 16: 182–192 |
| Immunogen | CDC2 (Phospho-Tyr15) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y (p) -G-V) derived from Human CDC2. |
| Modification | p-Tyr15 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK1 |
|---|---|
| Alternative Names | CDC2 CDC28A P34CDC2 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A CDC28 CDC2A CDK1 Cyclin-dependent kinase 1 |
| Molecular Weight(MW) | 34 kDa |
| Tissue Specificity | This antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15. |
| Entrez Gene | 983 |
|---|---|
| Protein Accession | NP_001163877.1 |
Application
Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Tyr15).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
