Application
Western blot analysis of lysed extracts from HeLa and 3T6 cells using cdc25A (Ab-76).| Product Name | cdc25A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25A2, CDC25M3, MPIP1 |
| Product description | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.1) Jin J, et al. (2003) Genes 17 (24): 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14): 3694-703. |
| Immunogen | cdc25A (Ab-76) antibody was raised against a peptide sequence around aa. 73~77 (M-G-S-S-E) derived from Human cdc25A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25A |
|---|---|
| Alternative Names | CDC25A2 CDC25M3 MPIP1 |
| Molecular Weight(MW) | 70 kDa |
| Tissue Specificity | This antibody detects endogenous level of total cdc25A protein. |
| Entrez Gene | 993 |
|---|---|
| Protein Accession | NP_001780.2 |
Application
Western blot analysis of lysed extracts from HeLa and 3T6 cells using cdc25A (Ab-76).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
