Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).| Product Name | cdc25A (phospho Ser76) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25A2, MEK1, PRKMK1, CDC25M3, MPIP1 |
| Product description | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.1) Jin J, et al. (2003) Genes 17 (24): 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14): 3694-703. |
| Immunogen | cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A. |
| Modification | p-Ser76 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25A |
|---|---|
| Alternative Names | CDC25A2 MEK1 PRKMK1 CDC25M3 MPIP1 |
| Molecular Weight(MW) | 70 kDa |
| Tissue Specificity | This antibody detects endogenous level of cdc25A only when phosphorylated at serine 76. |
| Entrez Gene | 993 |
|---|---|
| Protein Accession | NP_001780.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
