Application
Immunohistochemistry staining of CDC25A in testis tissue using CDC25A Antibody.| Product Name | CDC25A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25A, CDC25A2-CAG isoform, Cell division cycle 25A, CDC25A phosphatase, CDC25Hu1, M-phase inducer phosphatase 1, CDC25A2 |
| Product description | CDC25A, a CDC25 dual specificity protein phosphatase, has been shown to activate the cyclin-dependent kinases at different points of the cell cycle. CDC25A has been shown to affect S phase entry and possibly the initiation of mitosis.CDC25A dephosphorylates cyclin dependent kinases, including Cdk2, Cdk4, as well as non cyclin proteins such as the homeodomain transcription factor cut, the proto-oncogene Raf-1. CDC25A has been implicated in many carcinomas, including those of the colon, lymph node, stomach, ovary, breast. CDC25A is located on chromosome 3p21 in an area frequently involved in karyotypic abnormalities in renal carcinomas, small cell carcinomas of the lung, and benign tumors of the salivary gland. |
| Immunogen | CDC25A antibody was raised against amino acids surrounding Ser 75 of CDC25A (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| CDC25A antibody can be used in ELISA, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:100, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25A |
|---|---|
| Alternative Names | CDC25A CDC25A2-CAG isoform Cell division cycle 25A CDC25A phosphatase CDC25Hu1 M-phase inducer phosphatase 1 CDC25A2 |
| Tissue Specificity | pSer75 |
Application
Immunohistochemistry staining of CDC25A in testis tissue using CDC25A Antibody.| Application Notes | CDC25A antibody can be used in ELISA, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:100, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store CDC25A antibody at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. |
