CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.1) Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004).
Immunogen
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Cdc25A.
Western blot analysis of Cdc25A Pab in mouse kidney tissue lysates (35ug/lane)
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.