Application
Western Blot at 1:2000 dilution + HCT116 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with Cdc25A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.| Product Name | Cdc25A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A |
| Product description | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.1) Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). |
| Immunogen | This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25A |
|---|---|
| Alternative Names | M-phase inducer phosphatase 1 Dual specificity phosphatase Cdc25A CDC25A |
| Molecular Weight(MW) | 59 kDa |
Application
Western Blot at 1:2000 dilution + HCT116 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with Cdc25A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
