Application
Western blot analysis of extracts from COS7 cells treated with Etoposide using Phospho-CDC25B (Ser353) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | CDC25B (phospho Ser353) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25M2, MPIP2, Dual specificity phosphatase Cdc25B, CDC25HU2, |
| Product description | Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.1) Reboutier D, et al. (2012) J Cell Biol 197, 19-26 |
| Immunogen | CDC25B (phospho Ser353) antibody was raised against a peptide sequence around phosphorylation site of serine 353 (R-R-S (p)-V-T) derived from Human CDC25B . |
| Modification | p-Ser353 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25B |
|---|---|
| Alternative Names | CDC25M2 MPIP2 Dual specificity phosphatase Cdc25B CDC25HU2 |
| Molecular Weight(MW) | 64 kDa |
| Tissue Specificity | The antibody detects endogenous level of CDC25B only when phosphorylated at serine 353. |
| Entrez Gene | 994 |
|---|---|
| Protein Accession | NP_001274445.1 |
Application
Western blot analysis of extracts from COS7 cells treated with Etoposide using Phospho-CDC25B (Ser353) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
