Application
Western Blot in Ramos cell line lysates (35ug/lane). CDC25B(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDC25B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CDC25B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2 |
| Product description | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.1) Uchida, S., et al., Biochem. Biophys. Res. Commun. 316(1):226-232 (2004). |
| Immunogen | This CDC25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the Central region of human CDC25B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25B |
|---|---|
| Alternative Names | M-phase inducer phosphatase 2 Dual specificity phosphatase Cdc25B CDC25B CDC25HU2 |
| Molecular Weight(MW) | 65 kDa |
Application
Western Blot in Ramos cell line lysates (35ug/lane). CDC25B(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDC25B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
