Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDC25C (Phospho-Ser198) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | CDC25C (phospho Ser198) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25M1, MPI1B, MPIP3, EC 3.1.3.48 |
| Product description | cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.1) Sadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990). |
| Immunogen | CDC25C (phospho Ser198) antibody was raised against a peptide sequence around phosphorylation site of Serine 198 (E-F-S (p)-L-K) derived from Human CDC25C. |
| Modification | p-Ser198 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25C |
|---|---|
| Alternative Names | CDC25M1 MPI1B MPIP3 EC 3.1.3.48 |
| Molecular Weight(MW) | 53 kDa |
| Tissue Specificity | The antibody detects endogenous levels of CDC25C only when phosphorylated at serine 198. |
| Entrez Gene | 995 |
|---|---|
| Protein Accession | NP_001274511.1 |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDC25C (Phospho-Ser198) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
