Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using cdc25C (Phospho-Ser216).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using cdc25C (Phospho-Ser216).| Product Name | cdc25C (phospho Ser216) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25, PPP1R60, MACS, PRKCSL, CDC25M1, MPIP3 |
| Product description | cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.1) Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3 (4): 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25 (7): 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4 (7): 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99 (4): 1990-1995. |
| Immunogen | cdc25C (Phospho-Ser216) antibody was raised against a peptide sequence around phosphorylation site of serine 216 (S-P-S (p) -M-P) derived from Human cdc25C. |
| Modification | p-Ser216 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25C |
|---|---|
| Alternative Names | CDC25 PPP1R60 MACS PRKCSL CDC25M1 MPIP3 |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of cdc25C only when phosphorylated at serine 216. |
| Entrez Gene | 995 |
|---|---|
| Protein Accession | NP_001781.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using cdc25C (Phospho-Ser216).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using cdc25C (Phospho-Ser216).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
