Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | CDC25C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25C, CDC25, PPP1R60 |
| Product description | CDC25C gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.1) Bonnet, J., (2008) Biochem. Biophys. Res. Commun. 370 (3), 483-488. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDC25C. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CDC25C antibody can be used for detection of CDC25C by ELISA at 1:62500. CDC25C antibody can be used for detection of CDC25C by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC25C |
|---|---|
| Alternative Names | CDC25C CDC25 PPP1R60 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | CDC25C antibody can be used for detection of CDC25C by ELISA at 1:62500. CDC25C antibody can be used for detection of CDC25C by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CDC25C antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
