Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
CDC37 Antibody IHC analysis in formalin fixed and paraffin embedded skin followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of CDC37 Antibody in MCF-7, A2058 cell line lysates (35ug/lane)Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | CDC37 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit, p50Cdc37, Hsp90 co-chaperone Cdc37, N-terminally processed, CDC37, CDC37A |
| Product description | CDC37 is a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.1) Dai,K., et.al., J. Biol. Chem. 271 (36), 22030-22034 (1996) |
| Immunogen | This CDC37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the Central region of human CDC37. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC37 |
|---|---|
| Alternative Names | Hsp90 co-chaperone Cdc37 Hsp90 chaperone protein kinase-targeting subunit p50Cdc37 Hsp90 co-chaperone Cdc37 N-terminally processed CDC37 CDC37A |
| Molecular Weight(MW) | 44 kDa |
Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
CDC37 Antibody IHC analysis in formalin fixed and paraffin embedded skin followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of CDC37 Antibody in MCF-7, A2058 cell line lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
