Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CDC5L Antibody in MCF-7 cell line lysates (35ug/lane)| Product Name | CDC5L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein, CDC5L, KIAA0432, PCDC5RP |
| Product description | The protein is a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.1) Grillari,J., et.al., J. Biol. Chem. 284 (42), 29193-29204 (2009) Zhang,N., et.al., EMBO Rep. 10 (9), 1029-1035 (2009) |
| Immunogen | This CDC5L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-213 amino acids from the Central region of human CDC5L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC5L |
|---|---|
| Alternative Names | Cell division cycle 5-like protein Cdc5-like protein Pombe cdc5-related protein CDC5L KIAA0432 PCDC5RP |
| Molecular Weight(MW) | 92 kDa |
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CDC5L Antibody in MCF-7 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
