Application
Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | CDK1/CDC2 (phospho Thr14) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC28, CDC2A, CDK1, MPF, kinase Cdc2 |
| Product description | Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.1) Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49 |
| Immunogen | CDK1/CDC2 (phospho Thr14) antibody was raised against a peptide sequence around phospho rylation site of threonine14 (E-G-T (p)-Y-G) derived from Human CDK1/CDC2 . |
| Modification | p-Thr14 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK2 |
|---|---|
| Alternative Names | CDC28 CDC2A CDK1 MPF kinase Cdc2 |
| Molecular Weight(MW) | 34 kDa |
| Tissue Specificity | The antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14. |
| Entrez Gene | 1017 |
|---|---|
| Protein Accession | NP_001277159.1 |
Application
Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
