Application
Flow cytometric analysis of jurkat cells using CDK2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of anti-CDK2 Antibody in Jurkat cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CDK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2 |
| Product description | CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.1) Moshinsky, D.J., et al., Biochem. Biophys. Res. Commun. 310(3):1026-1031 (2003). |
| Immunogen | This CDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the C-terminal region of human CDK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CDK2 |
|---|---|
| Alternative Names | Cyclin-dependent kinase 2 Cell division protein kinase 2 p33 protein kinase CDK2 CDKN2 |
| Molecular Weight(MW) | 34 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of jurkat cells using CDK2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of anti-CDK2 Antibody in Jurkat cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
