Application
Immunofluorescence of CDKN1C in 293 cells with CDKN1C antibody at 20 μg/mL.Application
Western blot analysis of CDKN1C in 293 cell lysate with CDKN1C antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CDKN1C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN1C Antibody: BWS, WBS, p57, BWCR, KIP2, p57Kip2, Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57 |
| Product description | CDKN1C Antibody: The cyclin-dependent kinase inhibitor 1C (CDKN1C), also known as p57KIP2, is a tight-binding, strong inhibitor of several G1 cyclin/CDK complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndrome, a cancer-predisposing syndrome, suggesting that this gene is a tumor suppressor candidate. CDKN1C is widely expressed during organogenesis with its expression declining after maturity. It is part of the CIP/KIP family of cyclin-dependent inhibitors (CKIs) which also includes CDKN1A and CDKN1B.1) Lee MH, Reynisdottir I, and Massague J. Cloning of p57KIP2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 1995; 9:639-49. |
| Immunogen | CDKN1C antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDKN1C. The immunogen is located within amino acids 40 - 90 of CDKN1C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CDKN1C antibody can be used for detection of CDKN1C by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDKN1C |
|---|---|
| Alternative Names | CDKN1C Antibody: BWS WBS p57 BWCR KIP2 p57Kip2 Cyclin-dependent kinase inhibitor 1C Cyclin-dependent kinase inhibitor p57 |
| Molecular Weight(MW) | 35 kDa |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | At least three isoforms of CDKN1C are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, CDKN1C often migrates at 57kDa in SDS-PAGE. CDKN1C antibody is predicted to not cross react with other CIP/KIP proteins. |
Application
Immunofluorescence of CDKN1C in 293 cells with CDKN1C antibody at 20 μg/mL.
Application
Western blot analysis of CDKN1C in 293 cell lysate with CDKN1C antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | CDKN1C antibody can be used for detection of CDKN1C by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CDKN1C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CDKN1C Antibody is supplied in PBS containing 0.02% sodium azide. |
