Application
Immunofluorescence of CDNF in Human Brain cells with CDNF antibody at 20 μg/mL.Application
Immunohistochemistry of CDNF in human brain tissue with CDNF antibody at 5 μg/mL.Application
Western blot analysis of CDNF in A-20 cell lysate in (A) the absence and (B) the presence of blocking peptide with CDNF antibody at 1 μg/mL.| Product Name | CDNF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CDNF Antibody: The conserved dopamine neurotrophic factor (CDNF) is a neurotrophic factor for dopaminergic neurons and highly homologous to the mesencephalic-astrocyte-derived neuro-trophic factor. Somewhat surprisingly, CDCF is expressed at higher levels in tissues such as heart, skeletal muscle and testes than in brain. Similar to the glial cell line-derived neurotrophic factor (GDNF), CDNF can prevent the 6-hydroxylamine (6-OHDA)-induced degeneration of dopaminergic neurons in a rat model of Parkinson's disease. Furthermore, CDNF was able to restore the dopaminergic function and prevent the degeneration of dopaminergic neurons in the substantia nigra, suggesting that CDNF might be suitable for the treatment of Parkinson's disease. At least two isoforms of CDNF are known to exist.1) Lindholm P, Voutilainen MH, Lauren J, et al. Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo. Nature2007; 448:73-7. |
| Immunogen | CDNF antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human CDNF. The immunogen is located within the first 50 amino acids of CDNF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CDNF antibody can be used for detection of CDNF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARMETL1 |
|---|---|
| Alternative Names | CDNF Antibody: ARMETL1 ARMETL1 Cerebral dopamine neurotrophic factor ARMET-like protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody does not cross-react with MANF. |
Application
Immunofluorescence of CDNF in Human Brain cells with CDNF antibody at 20 μg/mL.
Application
Immunohistochemistry of CDNF in human brain tissue with CDNF antibody at 5 μg/mL.
Application
Western blot analysis of CDNF in A-20 cell lysate in (A) the absence and (B) the presence of blocking peptide with CDNF antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | CDNF antibody can be used for detection of CDNF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
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| Storage Instructions | CDNF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CDNF Antibody is supplied in PBS containing 0.02% sodium azide. |
